M-MLV Reverse Transcriptase(200U/μL)
M-MLV Reverse Transcriptase(200U/μL)
Stored at: -20℃
REF GSP9001 Spec: 50μL(10000U)
GSP9002 100μL(20000U)
GSP9003 500μL(40000U)
GSP9004 1000μL(200000U)
M-MLV Reverse Transcriptase is derived from the Moloney Murine Leukemia Virus, a retrovirus that infects mice. This enzyme is a DNA polymerase that exhibits reverse transcriptase activity, meaning it catalyzes the synthesis of cDNA from RNA template. M-MLV Reverse Transcriptase enables analysis of gene expression,discovery of novel RNA sequences,and investigation of RNA - based regulatory mechanisms. ABIOM M-MLV RT is a new reverse transcriptase with high sensitivity developed by ABIOM.
Features
Resistant to 65 ℃, suitable for high GC and RNA templates with complex secondary structures
Suitable for reverse transcription of a small number of templates and low-copy genes
Capable of expanding and growing up to 10kb of cDNA
RNaseH activity free reduces reverse RNA degradation
Applications
Construction of a full-length cDNA library
Endpoint PCR
Real time quantitative PCR
Performance
1. SINOVA M-MLV RT has equivalent or superior performance than well-known domestic brand Q
2. Withstand high temperature of 50 ℃
3. SINOVA M-MLV RT has equivalent or superior performance than well-known domestic brand N
Fig. 4-fold RT qPCR detection
4. The main peak of sequencing is consistent, and the performance of SINOVA M-MLV RT is equivalent to brand S
5. High protein purity≥95%
6. Excellent stability
Fig. SINOVA M-MLV RT activity did not change significantly after storage at 4 ℃ for one month
7. No DNase activity
Fig. Under normal usage, the residual amount of SINOVA M-MLV RT DNase is far lower than 0.1U/μL (higher than 0.1U has a significant impact on one-step RT qPCR reaction).
8. RNase residue experiment
Fig. Under normal usage, the residual amount of SINOVA M-MLV RT RNase is far lower than 100pg(higher than 800pg has a significant impact on one-step RT qPCR reaction)
9. Endotoxin residue test
Fig. The endotoxin residue of SINOVA M-MLV RT was lower than 0.06EU/μL
10. SINOVA M-MLV RT reverse transcription extension performance is equivalent to or even better than internationally renowned brand S
Fig. One step RT-qPCR Reaction method for determining reverse transcription performance
Fig. Determination of reverse transcription elongation length by two step RT-PCR Agarose gel Electrophoresis
11. The high-temperature resistance of reverse transcription is equivalent to or even better than internationally renowned brand S
Fig. Determination of reverse transcription tolerance temperature by two-step RT-PCR agarose gel electrophoresis
