Taq-Hs DNA Polymerase(5U/μL)
Taq-Hs DNA Polymerase(5U/μL)
Stored at: -20℃
REF GSP9121 Spec: 50μL(250U)
GSP9122 100μL(500U)
GSP9123 500μL(2500U)
GSP9124 1000μL(5000U)
This product is a type of enzyme that enhances the specificity and sensitivity of PCR reactions. This kind of enzyme is modified by antibody method to DNA polymerase, so that DNA polymerase activity can be activated at the pre denaturation temperature, which can inhibit the non-specific annealing of primers or the non-specific amplification caused by primer dimers under low temperature conditions.
Features
High purity, no residual nucleic acid exonuclease, incision enzyme, or RNase
Low temperature inhibition and high temperature activation increase the specificity of DNA amplification
Applications
PCR, qPCR and RT-qPCR
Genetic testing
SNP typing
Performance
1. Complete inhibition of non-specific amplification at room temperature (25 ℃)
Fig: The activation of SINOVA's Taq-Hs DNA Polymerase at 25 ℃ can effectively inhibit non-specific amplification, while ordinary Taq exhibits more significant non-specific amplification
2. High efficiency of multiple gene amplification
Fig. SINOVA's Taq-Hs DNA Polymerase can simultaneously amplify multiple different genes, and the amplification efficiency is higher or equal to that of foreign T companies, with wide gene adaptability and high efficiency
3. TaqMan detection - SINOVA Taq-Hs DNA Polymerase amplification with longer fragments and faster extension speed
Fig. SINOVA three well has good repeatability, small fluctuations, and high amplification efficiency, while domestic N company has large fluctuations in three well duplication, low amplification efficiency, and foreign T company does not amplify at all
4. Good thermal stability
Fig. After 15 days of storage at 37 ℃, triple gene amplification was performed. Compared with the control at -20 ℃, the CT value and fluorescence intensity were close to those of the control, and there was no change in activity. SINOVA's Taq-Hs DNA Polymerase had a longer storage time at 37 ℃
